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Image Search Results
Journal: Cell Communication and Signaling : CCS
Article Title: LRRK2 is involved in the chemotaxis of neutrophils and differentiated HL-60 cells, and the inhibition of LRRK2 kinase activity increases f MLP-induced chemotactic activity
doi: 10.1186/s12964-023-01305-y
Figure Lengend Snippet: Lrrk2 knockdown results in a decrease in OXPHOS activities of dHL-60 cells. A , B dHL-60 cells expressing tetracycline-inducible Lrrk2 shRNA or irrelevant (Irr) shRNA were cultured in 1.25% DMSO with 0.5 µg/ml doxycycline (Dox) for 3 days. The OXPHOS activities of these cells were measured after treatment with 1 µM f MLP for 5 min ( n = 4). Error bars, SEM. A complex I. B complex I + II. C Representative immunofluorescence microscope images of mitochondria. dHL-60 cells expressing tetracycline-inducible Lrrk2 shRNA or irrelevant (Irr) shRNA were cultured in 1.25% DMSO with 0.5 µg/ml Dox for 3 days. dHL-60 cells attached to coverslips were then incubated with or without 10 nM f MLP for 5 min. Green; anti-TOMM20, Red; anti-ß-actin, Blue; DAPI. Bar, 10 µm. D Percentages of cells with mitochondria of tubular form after f MLP stimulation. > 50 cells were analyzed in four independent experiments. Error bars, SEM. ** p < 0.01 compared with Irr shRNA expressing dHL-60 cells
Article Snippet: The following antibodies were used in this study:
Techniques: Expressing, shRNA, Cell Culture, Immunofluorescence, Microscopy, Incubation